乳化剂HLB值对莪术油亚微乳理化性质的影响

论文编号:SW002  论文字数:9937,页数:19

摘要：目的 制备莪术油亚微乳，并考察乳化剂的HLB值对亚微乳理化性质的影响。方法 使用高压微射流纳米分散仪制备含不同HLB值乳化剂的莪术油亚微乳；激光粒度及电泳分析仪测定其粒径和表面电位；离心法测定其稳定常数；测定药物在不同HLB值乳化剂的水相中的溶解度，并采用透析法测定亚微乳的包封率和体外释放。结果制备得到的亚微乳粒径在123~154 nm 之间，粒子带负电。几种乳化剂对药物均有明显的增溶作用，增溶倍数从5.5~8.2倍不等，药物包封率为73～83%，体外释放符合Higuchi方程，且能持续释放48小时。结论 乳化剂的HLB值不同，制备得到的亚微乳的性质如粒径和稳定性有所不同；随着HLB值增加，乳化剂对药物的增溶作用增强，药物包封率略有下降，但体外释放行为没有显著差。

关键词：HLB值；亚微乳；莪术油；稳定性；包封率；体外释放

Abstract：Objective  To prepare curcuma zedoaria oil-loaded submicron emulsion and investigate the effect of HLB on its physicochemical properties. Methods The high pressure homogenization method was employed to prepare curcuma zedoaria oil-loaded submicron emulsion. The particle distribution and zeta-potential were determined by using zetasizer. The stability was investigated by centrifugalization. The solubilities of zedoaria oil in aqueous phase with various HLB value were determined.  The dialysis method was established for determination of entrapment efficiency and drug release behavior of curcuma zedoaria oil in the submicron emulsion. Results Curcuma zedoaria oil-loaded submicron emulsion was charge negative and with the mean particle size between 123 nm and 154 nm. Solubilizations were observated obviously with emulsifiers of various HLB value, and solubilization multiples were between 5.5  and 8.2. The drug encapsulation efficiencies of curcuma zedoaria oil-loaded submicron emulsion were between 73% and 83%. In vitro drug release experiments indicated the drug release from submicron emulsion was lasted to 48 hours, and the release behavior was consistent with Higuchi equation. Conclusion The curcuma zedoaria oil-loaded submicron emulsions with various HLB value of emulsifier were prepared. Their physicochemical properties such as particle distribution and stability were different accompany with various HLB value of emulsifiers. And as HLB value of emulsifiers increased, more solubilizations were observated, thus entrapment efficiencies were decreased slightly But no significant deviations were observated when released in vitro, and was represented a sustained release.
Key words：HLB value; submicron emulsion; curcuma zedoaria oil; stability; entrapment efficiency; drug release

目  录
中文摘要 I
英文摘要 II
目录 III
1. 绪论 1
 1.1 莪述油的基本介绍 1
      1.2 莪述油的提取 1
 1.3 莪述油的化学成分 1
 1.4 莪术油的药理作用 2
 1.5 莪术油制剂的临床应用 2
 1.6 莪述油乳剂的制备方法 4
2. 实验部分 5
 2.1 仪器与药品 5
 2.2 实验方法 5
3. 结果与讨论 7
 3.1 粒径和电位 7
    3.2 稳定性 7
 3.3 药物包封率 8
 3.4 体外释放 8
 3.5 讨论 11
4．总结与展望 12
致谢 14
参考文献 15

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