茯苓多糖提取及氧化研究

论文编号:SW037  论文字数:10290,页数:25

 摘 要：以茯苓菌核为原料，在超声波辅助下，深讨了碱溶性茯苓多糖的提取条件。用2，2，6，6-四甲基哌啶-1-氧自由基（TEMPO）将茯苓多糖进行氧化，制得氧化茯苓多糖，用红外光谱进行了表征，并用胆酸结合能力的测定来比较多糖及氧化产物的降低胆固醇的能力,结果表明氧化后的茯苓多糖结合胆酸的能力比茯苓多糖下降。
 
 关键词：茯苓多糖；提取；TEMPO；胆酸结合试验
 
Studies on the Extraction and Oxidation of Poria cocos Polysaccharides

Abstract: Alkali-soluble parchymaran was extracted by using dilute alkali (0.5mol/l NaOH) solution as a solvent with the assistance of ultrasonic and the extraction condition was examined. 2,2,6,6-Tetramethyl-1-piperidine oxoammonium ion (TEMPO)-mediated oxidation was applied to parchymaran. and the physicochemical properties of the resulting derivatives were investigated by using FT-IR and bile acid binding activity. The oxidized derivative showed lower bile acid binding activity than native glucan , it could be explained by the stronger interaction of carboxyl groups and bile acid .
 
Keywords: polysaccharides; extraction; ultrasonic wave; oxidation.

目  录
中文摘要 I
英文摘要 II
目录 III
1、绪论 1
1.1 茯苓的简介 1
1.2 茯苓多糖的结构及作用 1
1.3 茯苓多糖氧化后的作用 2
1.4 茯苓多糖的提取 2
1.5 粗多糖的纯化 5
1.6 茯苓多糖的氧化 5
1.7 茯苓多糖及其衍生物体外生物活性的检测 6
1.8 实验思路 6
2、实验部分 7
2. 材料试剂与设备 7
2.2 实验步骤 7
3 结果与讨论 11
3.1 波长的选择 11
3.2 标准曲线的制作 11
3.3 单因素分析 12
3.4 茯苓多糖及其氧化衍生物的红外谱图 15
3.5 胆酸结合能力试验 17
4、结论 18
4.1 结论 18
4.2 展望 18
致   谢 19
参考文献 20

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