猪肉中药物残留的检测

论文编号:SW043  论文字数:11751,页数:29

摘 要：随着经济的发展，膳食结构的不断改善，动物性食品的需求不断增加。兽药在降低动物发病率与死亡率，提高饲料利用率，促进生长和改善产品品质等方面起着非常重要的作用。但是，由于管理不当和受经济利益驱使，各种兽药被非法大量添加，造成肉类中不同程度的兽药残留，对消费者的健康产生了各种危害。本论文研究的内容为采用的检测方法为ELISE法，运用适当的前处理，再使用试剂盒，通过酶联反应，对脱水猪肉干进行残留药物检测。其主要项目为检测猪肉中克伦特多、氯霉素、呋喃它酮和呋喃唑酮的残留量。由于呋喃它酮和呋喃唑酮在体内易被代谢，所以在分析此类药物时采用分析此药物的代谢产物AMOZ和AOZ。经试验，本实验所检验的20批猪肉样品中兽药残留均符合标准，未出现超标现象。

关键词：猪肉；药物残留；酶联反应；检测

Study and detection of drug residues in Pork

Abstract:
 With the development of economic and improvement of diet,the d-emand for animal products is increasing.Animal veterinary medicine  have an effect on reducing morbidity,improving feeding efficiency,pr-omoting growth and improving production quality.However,due to mi-smanagement and driven by economic interests,a large number of vet-erinary drugs are added to animal feed,resulting veterinary drug resid-ues with different levels,causing badly health hazards to consumers.T-he most important detection method used in this paper is the ELISE,which by using appropriate pre-processing, react in the Kits,detect thedrug residues of dry pork.The main testing contains the residues of  clenbuterolchloramphenicol,furazolidone and furaltadone.As furaltadoneand furazolidone are susceptible to metabolism,so while dealing with these drugs used in the analysis the metabolites of this drug which  is called AOZ and AMOZ. The results show,these 20 samples of ap-proved veterinary drug residues in pork are standard,the phenomenon of residues more than the standard haven't appeared.

Keywords: Pork;drug residues;ELISE;detection

目  录
中文摘要 I
英文摘要 II
目  录 III
1 绪论 1
1.1 克伦特罗 2
1.2 氯霉素 3
1.3 硝基呋喃类药物 4
1.4 食品检测的意义和展望 5
2 实验内容 6
2.1 实验材料 6
2.2 实验方法 8
3 实验结果与讨论 15
3.1 各种试剂盒中标准液的标准曲线 15
3.2 实验结果 19
3 实验结论 22
致  谢 23
参考文献 24

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