乳清小肽免疫调节作用及抗过敏功效初步实验

论文编号:SP084  论文字数:10254,页数:22

摘 要：用18-22g的ICR小鼠进行碳粒廓清试验和血清溶血素测定，用18-22g的BALB/c小鼠进行淋巴细胞增殖反应试验和NK细胞活性试验，用180-220g的SD大鼠进行被动皮肤过敏试验（PCA）。观察乳清小肽对小鼠碳粒吞噬指数、小鼠血清半数溶血值的影响，观察乳清小肽对小鼠淋巴细胞增殖反应的刺激指数和NK细胞活性的影响，观察乳清小肽对被动皮肤过敏大鼠的蓝斑面积、蓝斑OD值的影响。
 试验结果表明，乳清小肽对免疫有调节作用；能轻微增强小鼠单核巨噬细胞系统的功能，具有轻微的增强小鼠的非特异性免疫功能的作用，乳清小肽可抑制SRBC免疫小鼠的溶血素抗体的产生，抑制小鼠的特异性体液免疫功能；能抑制小鼠淋巴细胞的增殖反应，抑制小鼠的特异性细胞免疫功能；并且能抑制大鼠被动皮肤过敏反应的作用。
关键词：乳清小肽，碳粒廓清试验，血清溶血素测定，淋巴细胞增殖反应试验，NK细胞活性试验，PCA

Abstract: The experiment is tested with 18-22g of ICR mice carbon clearance test and serum hemolysin, with 18-22g of BALB / c mice lymphocyte proliferation and NK cell activity test, with 180-220g of SD rats were passive cutaneous anaphylaxis test (PCA).Observing the Whey peptides’ impacts on carbon phagocytic index in mice and serum hemolysin.meanwhile,obtaining the results through the  observation of whey peptides on lymphocyte proliferation stimulation index and NK cell activity,and the Observation of whey peptides on the passive cutaneous anaphylaxis in rat locus coeruleus area of OD value of blue spots.
The results show that whey peptides has the effects on the immune regulatory; can slightly enhance the mouse monocyte-macrophage system function, mice with mild non-specific enhancement of immune function,whey peptide can inhibit the immune SRBC hemolysin antibodies, inhibition of specific humoral immune function in mice; can inhibit the proliferation of lymphocytes, inhibition of specific cellular immune function in mice; and could inhibit the role of passive cutaneous anaphylaxis.
Keywords:Whey peptides; Carbon clearance test; Determination of serum hemolysin; Lymphocyte proliferation test; NK cell activity tests; PCA
 

目  录
中文摘要 I
英文摘要 II
目录 III
1. 绪论 1
 1.1  乳清小肽 2
 1.2实验目的 2
2. 实验过程 2
 2.1 试验目的 2
 2.2受试药物   2
 2.3试验动物 2
 2.3.1大鼠 2
 2.3.2小鼠 2
 2.3.3豚鼠 2
 2.4实验条件 3
 2.5剂量组别 3
 2.5.3给药途径与剂量 3
 2.6主要仪器与试剂 3
 2.7实验方法 5
 2.8观察指标 8
 2.9统计学分析 8
3. 结果与讨论 8
 3.1实验结果 8
 3.2讨论 14
4.结论与展望 15
 4.1结论 15
 4.2展望 15 
致谢 17
参考文献 18

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