蔬菜中农药残留检测方法的研究

论文编号:ZY023    论文字数:9735,页数:22

摘要：本文建立了蔬菜中多种农药残留同时检测的毛细管气相色谱分析法，完成了蔬菜中有机磷、有机氯等17种农药残留的样品前处理及色谱分析方法研究。有机磷经丙酮提取，凝胶层析柱净化，HP-5石英毛细管色谱柱分离，火焰光检测器检测。有机氯类用乙酸乙酯—环己烷和凝胶净化柱提取净化，石英毛细管气相色谱（配ECD检测器）检测。实验结果表明，17种有机磷、有机氯类农药残留均获得良好分离，样品的加标回收率为79%- 110.14%，相对标准偏差为3.14%-11.69%,农药残留量色谱分析的线性关系良好满足定量分析要求。本法因其样品处理方便、净化效果好，方法准确、可靠，其精密度、准确度均能满足分析的要求,对蔬菜安全研究、过程分析及无公害蔬菜评价都具有广阔的应用前景。
关键词：有机磷;有机氯;农药残留;气相色谱

Abstract: This artical established the capillary GC analytica method in surveying manifold pesticides in vegetable at one time. completed the vegetables of organic phosphorus, organic chlorine 17 pesticide residues in the sample pre-treatment and chromatography analysis. Organic phosphorus by acetone extraction, gel column chromatography purification, HP-5 silica capillary column chromatography, flame-ray detector. Organic chlorine using ethyl acetate - cyclohexane and gel purification column extraction purification, quartz capillary gas chromatography (ECD allocation) detection. Experimental results show that 17 species of organic phosphorus, organic pesticides residues were well separated, samples in the range of 79% - 110.14%. The relative standard deviation of 3.14% -11.69% Pesticide residues chromatography analysis of the linear relationship was good to meet quantitative analysis requirements. Act because the sample processing, effective purification method is accurate, reliable, precision, accuracy can meet the requirements analysis, Vegetable security research, analysis and evaluation of pollution-free vegetables have broad application prospects.
Keywords: Organic phosphorus; Organic chlorine; Pesticide residues; GC

目录
摘要……………………………………………………………………………………Ⅰ
Abstract………………………………………………………………………………Ⅱ
目录……………………………………………………………………………………Ⅲ
1 前言………………………………………………………………………………… 1
 1.1农药残留概述……………………………………………………………………1
 1.2 农产品农药残留快速检测现状……………………………………………… 2
 1.3 课题研究的目的和意义……………………………………………………… 4
 1.4 检测原理…………… …………………………………………………………4
2 实验部分………………… …………………………………………………………6
 2.1 材料和方法…………… ………………………………………………………6
 2.2 气相色谱分析条件…………… ………………………………………………7
 2.3 有机磷类农药检测样品前处理……………………… ………………………7
 2.4 有机氯类农药检测样品前处理………………………… ……………………8
3 结果与讨论………………………………………………………………………… 9
 3.1 有机磷类农药检测的结果与讨论………………………………………… …9
 3.2 有机氯类农药检测的结果与讨论…………………………………………… 12
 4 结论与展望………………………………………………………………… …… 16
致谢………………………………………………………………………………… …17
参考文献…………………………………………………………………………… …18

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