牛初乳中乳铁蛋白提取工艺的研究

论文编号:ZY030    论文字数:12126,页数:23

摘要：本论文研究的是牛初乳中乳铁蛋白的提取。牛初乳是牛3天内产的乳, 是自然界营养最为富集的食品资源之一。牛初乳含有很多活性物质,比如乳铁蛋白。 乳铁蛋白（Lf）的含量在初乳中最为丰富。Lf具有多种生物活性功能，如广谱抗菌活性，增强铁的传递和吸收，免疫调节作用，调节发炎反应，抗病毒作用，可广泛应用于食品，医疗和化妆品领域。但是就我国目前而言其中大部分的营养活性成分并没有得到充分利用而被浪费掉，因此如能加以充分利用将促进生物活性食品生产、具有明显的社会效益和经济效益。本课题通过离子交换层析法从天然产物初乳中纯化出乳铁蛋白。并考虑流速，柱高，进样浓度对收率的影响；用SDS-聚丙烯酰胺凝胶电泳、酶联免疫法和高效液相测纯度。
 
关键词：牛初乳；乳铁蛋白；电泳；酶联免疫法；提取

Abstract：This article research about the extraction of lactoferrin from bovine colostrums.Bovine colostrums is a kind of colostrums which  is gained from fist 3 days after cow’s breeding and one of the foods which contains much enrichment in natural. Bovine colostrum has lots of active elements,as lactoferrin for example.LF has various bioactive functions such as broad-spectrum  antimicrobial activity, promotion of iron transfer and absorption, immunomodulatory effects, regulation of inflammatory reactions, antibviral activity, and inhibition lf lipid peroxidation. but most of the active elements have not been fully used and wasted much in our country.As a rustle ,if we can make full use of Bovine colostrums, it will promote food production , has obvious social and economic benefits.LF was purified by ion exchange chromatography from bovine colostrums. At last it was measured by SDS-PAGE, Enzyme-linked immunosorbent assay and HPLC.

Keywords：Bovine colostrums；Lactoferrin；Electrophoresis；ELISA；Extraction

目  录
中文摘要……………………………………………………………………..I
英文摘要…………………………………………………………………….II
目录………………………………………………………….…………….III
目录………………………………………………………………………….IV
1. 绪论……………………………………………………………………….1
 1.1 牛初乳的生理功能和营养特征……………………………………..1
 1.2 乳铁蛋白……………………………………………………………..2
 1.3 乳铁蛋白的分离方法…………………………………………………5
 1.4 乳铁蛋白的检测方法…………………………………………………7
2.实验部分.………………………………………………………………….9
 2.1 树脂的选择……………………………………………………………9
 2.2 流速对离子交换吸附的影响…………………………………………11
 2.3 填料高度对离子交换吸附的影响…………………………………..12
 2.4SDS-PAGE凝胶电泳测定乳铁蛋白纯度……………………………..13
3. 结果与讨论……………………………………………………………..15
 3.1 树脂的选择结果与讨论…………………………………………………..15
 3.2流速对离子交换吸附的影响结果与讨论………………………….15
 3.3填料高度对离子交换吸附的影响结果和讨论…………………….15
 4．总结与展望……………………………………………………………..17
 4.1 实验总结…………………………………………………………….17
 4.2 展望………………………………………………………………….17
 致谢………………………………………………………………………….18
 参考文献…………………………………………………………………….19

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