Abstract:With using supercritical fluid carbon dioxide extraction technology ,onion oil were extracted from onion, and do the Antibacterial spectrum of research by Oxford cup method, and the medium dilution method were carried out on the bacteria minimal inhibitory concentration, minimum bactericidal concentration studies. The results of onion oil on the Staphyloccocus aureus , E. coli, Bacillus subtilis, yeast have a clear inhibitory effect ,but there is no inhibition on the effect of Aspergillus usamii,Aspergillus niger,Monascus purpureus,Blakeslea trispora,respectively.Corresp-
onding Staphyloccocus aureus , E. coli, Bacillus subtilis, yeast 1, the minimum inhibitory concentration for 0.00029,0.00036,0.00065,0.000072g/ ml, the minimum bactericidal concentration for 0.0014,0.00072,0.00072,0.000072 g/ ml.
Key Words: Supercritical fluid extraction;Onion oil; Antibacterial spectrum; Minimum inhibitory concentration; Minimum bactericidal concentration